› 2bc2

X-ray diffraction
1.7Å resolution

METALLO BETA-LACTAMASE II FROM BACILLUS CEREUS 569/H/9 AT PH 6.0, TRIGONAL CRYSTAL FORM

Released:
DOI: 10.2210/pdb2bc2/pdb
PDB entry 2bc2 coloured by chain, front view.
PDB entry 2bc2 coloured by chain, side view.
PDB entry 2bc2 coloured by chain, top view.
Source organism: Bacillus cereus
Entry authors: Fabiane SM, Sutton BJ

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-137499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.03 KDa
Source organism: Bacillus cereus
UniProt:
  • Canonical: P04190 (Residues: 31-257; Coverage: 100%)
Gene name: blm
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 5 x SO4
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3121
Unit cell:
a: 67.32Å b: 67.32Å c: 177.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.251

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