› 2bej

X-ray diffraction
2.1Å resolution

Structure of the bacterial chromosome segregation protein Soj

Released:
DOI: 10.2210/pdb2bej/pdb
PDB entry 2bej coloured by chain, front view.
PDB entry 2bej coloured by chain, side view.
PDB entry 2bej coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Bacterial chromosome segregation: structure and DNA binding of the Soj dimer--a conserved biological switch.
Leonard TA, Butler PJ, Löwe J
EMBO J 24 270-82 (2005)
PMID: 15635448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosome-partitioning ATPase Soj Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 27.57 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72H90 (Residues: 1-249; Coverage: 100%)
Gene names: TT_C1605, soj
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P43212
Unit cell:
a: 61.361Å b: 61.361Å c: 126.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

Assembly dynamics of the bacterial MinCDE system and spatial regulation of the Z ring.
Lutkenhaus J. (2007)
37 more

4 mentions without citation

The bacterial cytoskeleton.
Shih et al. (2006)
3 more