› 2bh8

X-ray diffraction
1.9Å resolution

Combinatorial Protein 1b11

Released:
DOI: 10.2210/pdb2bh8/pdb
PDB entry 2bh8 coloured by chain, front view.
PDB entry 2bh8 coloured by chain, side view.
PDB entry 2bh8 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A segment of cold shock protein directs the folding of a combinatorial protein.
de Bono S, Riechmann L, Girard E, Williams RL, Winter G
Proc Natl Acad Sci U S A 102 1396-401 (2005)
PMID: 15671167

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cold shock protein CspA Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 10.74 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9X9 (Residues: 1-36; Coverage: 51%)
Gene names: JW3525, b3556, cspA, cspS
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I422
Unit cell:
a: 101.09Å b: 101.09Å c: 76.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.192 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Evolution of protein fold in the presence of functional constraints.
Andreeva et al. (2006)
5 more

3 mentions without citation

Cold-Shock Domains-Abundance, Structure, Properties, and Nucleic-Acid Binding.
Heinemann et al. (2021)
2 more