› 2bhm

X-ray diffraction
2.4Å resolution

Crystal structure of VirB8 from Brucella suis

Released:
DOI: 10.2210/pdb2bhm/pdb
PDB entry 2bhm coloured by chain, front view.
PDB entry 2bhm coloured by chain, side view.
PDB entry 2bhm coloured by chain, top view.
Source organism: Brucella suis
Primary publication:
Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori.
Terradot L, Bayliss R, Oomen C, Leonard GA, Baron C, Waksman G
Proc Natl Acad Sci U S A 102 4596-601 (2005)
PMID: 15764702

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV secretion system protein virB8 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 164 amino acids
Theoretical weight: 18.22 KDa
Source organism: Brucella suis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CEG3 (Residues: 76-239; Coverage: 69%)
Gene names: BRA0062, BS1330_II0062, virB8
Sequence domains: VirB8 protein
Structure domains: VirB8 protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I4122
Unit cell:
a: 202.757Å b: 202.757Å c: 103.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.273
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
33 more

12 mentions without citation

Recent advances in the structural and molecular biology of type IV secretion systems.
Trokter et al. (2014)
11 more