› 2bry

X-ray diffraction
1.45Å resolution

Crystal structure of the native monooxygenase domain of MICAL at 1.45 A resolution

Released:
DOI: 10.2210/pdb2bry/pdb
Model geometry
Fit model/data
Data not analysed
PDB entry 2bry coloured by chain, front view.
PDB entry 2bry coloured by chain, side view.
PDB entry 2bry coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multidomain flavoenzyme-signaling molecule.
Siebold C, Berrow N, Walter TS, Harlos K, Owens RJ, Stuart DI, Terman JR, Kolodkin AL, Pasterkamp RJ, Jones EY
Proc Natl Acad Sci U S A 102 16836-41 (2005)
PMID: 16275925

Function and Biology Details

Reactions catalysed: 
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O
NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[F-actin]-monooxygenase MICAL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 55.24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VDP3 (Residues: 1-489; Coverage: 47%)
Gene names: Mical, Mical1, Nical
Sequence domains: FAD binding domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand CL 2 x CL
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 76.588Å b: 89.548Å c: 83.458Å
α: 90° β: 114.35° γ: 90°
R-values:
R R work R free
0.181 0.18 0.222
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Flavin dependent monooxygenases.
Huijbers et al. (2014)
15 more

7 mentions without citation

Actin filaments-A target for redox regulation.
Wilson et al. (2016)
6 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO