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GLU C180 -> GLN VARIANT QUINOL:FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES

X-ray diffraction
2.19Å resolution
PDB entry 2bs3 coloured by chain, front view.
PDB entry 2bs3 coloured by chain, side view.
PDB entry 2bs3 coloured by chain, top view.
Source organism: Wolinella succinogenes
Assembly composition:
hetero hexamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Fumarate reductase
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Multimeric state: hetero hexamer
Accessible surface area: 78821.27 Å2
Buried surface area: 38616.74 Å2
Dissociation area: 4,990.12 Å2
Dissociation energy (ΔGdiss): 40.75 kcal/mol
Dissociation entropy (TΔSdiss): 31.58 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-148052

Macromolecules

Fumarate reductase flavoprotein subunit
Chains: A, D
Length: 656 amino acids
Theoretical weight: 72.83 KDa
Source organism: Wolinella succinogenes
UniProt:
  • Canonical: P17412 (Residues: 1-656; Coverage: 100%)
Gene names: WS0831, frdA
Pfam:
InterPro:
CATH:
SCOP:
Fumarate reductase flavoprotein subunit in PDB entry 2bs3, assembly 1, front view.
Fumarate reductase flavoprotein subunit in PDB entry 2bs3, assembly 1, side view.
Fumarate reductase flavoprotein subunit in PDB entry 2bs3, assembly 1, top view.
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Fumarate reductase iron-sulfur subunit
Chains: B, E
Length: 239 amino acids
Theoretical weight: 27.2 KDa
Source organism: Wolinella succinogenes
UniProt:
  • Canonical: P17596 (Residues: 1-239; Coverage: 100%)
Gene names: WS0830, frdB
Pfam:
InterPro:
CATH:
SCOP:
Fumarate reductase iron-sulfur subunit in PDB entry 2bs3, assembly 1, front view.
Fumarate reductase iron-sulfur subunit in PDB entry 2bs3, assembly 1, side view.
Fumarate reductase iron-sulfur subunit in PDB entry 2bs3, assembly 1, top view.
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Fumarate reductase cytochrome b subunit
Chains: C, F
Length: 256 amino acids
Theoretical weight: 29.76 KDa
Source organism: Wolinella succinogenes
UniProt:
  • Canonical: P17413 (Residues: 1-256; Coverage: 100%)
Gene names: WS0832, frdC
Pfam: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
InterPro:
CATH: Fumarate reductase/succinate dehydrogenase, transmembrane subunit
SCOP: Fumarate reductase respiratory complex cytochrome b subunit, FrdC
Fumarate reductase cytochrome b subunit in PDB entry 2bs3, assembly 1, front view.
Fumarate reductase cytochrome b subunit in PDB entry 2bs3, assembly 1, side view.
Fumarate reductase cytochrome b subunit in PDB entry 2bs3, assembly 1, top view.
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