› 2bvl

X-ray diffraction
2.2Å resolution

Crystal structure of the catalytic domain of toxin B from Clostridium difficile in complex with UDP, Glc and manganese ion

Released:
DOI: 10.2210/pdb2bvl/pdb
PDB entry 2bvl coloured by chain, front view.
PDB entry 2bvl coloured by chain, side view.
PDB entry 2bvl coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
Structural basis for the function of Clostridium difficile toxin B.
Reinert DJ, Jank T, Aktories K, Schulz GE
J Mol Biol 351 973-81 (2005)
PMID: 16054646

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-538858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase TcdB Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 63.04 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18177 (Residues: 1-543; Coverage: 23%)
Gene names: tcdB, toxB
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MN 1 x MN
Ligand UDP 1 x UDP
Ligand GLC 1 x GLC
Ligand SO4 1 x SO4
Ligand TBR 4 x TBR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 63.01Å b: 95.797Å c: 112.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

27 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
26 more

21 mentions without citation

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity.
Wesolowski et al. (2009)
20 more