› 2c2p

X-ray diffraction
1.75Å resolution

The crystal structure of mismatch specific uracil-DNA glycosylase (MUG) from Deinococcus radiodurans

Released:
DOI: 10.2210/pdb2c2p/pdb
PDB entry 2c2p coloured by chain, front view.
PDB entry 2c2p coloured by chain, side view.
PDB entry 2c2p coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
The crystal structure of mismatch-specific uracil-DNA glycosylase (MUG) from Deinococcus radiodurans reveals a novel catalytic residue and broad substrate specificity.
Moe E, Leiros I, Smalås AO, McSweeney S
J Biol Chem 281 569-77 (2006)
PMID: 16223719

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.79 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RWF4 (Residues: 1-199; Coverage: 100%)
Gene name: DR_0715
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6
Unit cell:
a: 101.733Å b: 101.733Å c: 37.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.179 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Oxidative stress resistance in Deinococcus radiodurans.
Slade et al. (2011)
3 more

3 mentions without citation

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges.
Timmins et al. (2016)
2 more