› 2c4t

X-ray diffraction
2.3Å resolution

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with an inhibitor, an analogue of S- adenosyl methionine

Released:
DOI: 10.2210/pdb2c4t/pdb
PDB entry 2c4t coloured by chain, front view.
PDB entry 2c4t coloured by chain, side view.
PDB entry 2c4t coloured by chain, top view.
Source organism: Streptomyces cattleya
Entry authors: McEwan AR, Deng H, McGlinchey RP, Robinson DR, O'Hagan D, Naismith JH

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 299 amino acids
Theoretical weight: 32.4 KDa
Source organism: Streptomyces cattleya
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70GK9 (Residues: 1-299; Coverage: 100%)
Gene name: flA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SA8 3 x SA8
1 bound ligand:
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 76.319Å b: 129.505Å c: 182.845Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.239
Expression system: Escherichia coli BL21(DE3)

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