› 2c84

X-ray diffraction
2.31Å resolution

CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188 WITH CMP

Released:
DOI: 10.2210/pdb2c84/pdb
PDB entry 2c84 coloured by chain, front view.
PDB entry 2c84 coloured by chain, side view.
PDB entry 2c84 coloured by chain, top view.
Source organism: Pasteurella multocida
Primary publication:
Structural analysis of sialyltransferase PM0188 from Pasteurella multocida complexed with donor analogue and acceptor sugar.
Kim DU, Yoo JH, Lee YJ, Kim KS, Cho HS
BMB Rep 41 48-54 (2008)
PMID: 18304450

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-2,3/2,6-sialyltransferase/sialidase Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 45.53 KDa
Source organism: Pasteurella multocida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15KI8 (Residues: 25-412; Coverage: 94%)
Sequence domains: Sialyltransferase PMO188
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand C 1 x C
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU R-AXIS IV
Spacegroup: P212121
Unit cell:
a: 65.712Å b: 66.163Å c: 101.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Current trends in the structure-activity relationships of sialyltransferases.
Audry et al. (2011)
4 more