2c96

X-ray diffraction
1.8Å resolution

Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-153397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Psp operon transcriptional activator Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37344 (Residues: 1-265; Coverage: 82%)
Gene names: JW1296, b1303, pspF, ycjB
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P65
Unit cell:
a: 113.027Å b: 113.027Å c: 39.436Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.246
Expression system: Escherichia coli