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The crystal structure of Mycobacterium tuberculosis adenylate kinase in complex with two molecules of ADP and Mg2+ supports an associative mechanism for phosphoryl transfer.

Bellinzoni M, Haouz A, Graña M, Munier-Lehmann H, Shepard W, Alzari PM
Protein Sci 15 1489-93 (2006)
Cited: 24 times
EuropePMC logo PMID: 16672241

Abstract

The crystal structure of Mycobacterium tuberculosis adenylate kinase (MtAK) in complex with two ADP molecules and Mg2+ has been determined at 1.9 A resolution. Comparison with the solution structure of the enzyme, obtained in the absence of substrates, shows significant conformational changes of the LID and NMP-binding domains upon substrate binding. The ternary complex represents the state of the enzyme at the start of the backward reaction (ATP synthesis). The structure is consistent with a direct nucleophilic attack of a terminal oxygen from the acceptor ADP molecule on the beta-phosphate from the donor substrate, and both the geometry and the distribution of positive charge in the active site support the hypothesis of an associative mechanism for phosphoryl transfer.

Reviews - 2cdn mentioned but not cited (1)

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Articles - 2cdn mentioned but not cited (9)

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  4. Proteome scale comparative modeling for conserved drug and vaccine targets identification in Corynebacterium pseudotuberculosis. Hassan SS, Tiwari S, Guimarães LC, Jamal SB, Folador E, Sharma NB, de Castro Soares S, Almeida S, Ali A, Islam A, Póvoa FD, de Abreu VA, Jain N, Bhattacharya A, Juneja L, Miyoshi A, Silva A, Barh D, Turjanski A, Azevedo V, Ferreira RS. BMC Genomics 15 Suppl 7 S3 (2014)
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  6. Adenylate kinase from Streptococcus pneumoniae is essential for growth through its catalytic activity. Thach TT, Luong TT, Lee S, Rhee DK. FEBS Open Bio 4 672-682 (2014)
  7. Host-Induced Gene Silencing of an Adenylate Kinase Gene Involved in Fungal Energy Metabolism Improves Plant Resistance to Verticillium dahliae. Su X, Lu G, Li X, Rehman L, Liu W, Sun G, Guo H, Wang G, Cheng H. Biomolecules 10 E127 (2020)
  8. Structural and Biochemical Studies on the Reaction Mechanism of Uridine-Cytidine Kinase. Tomoike F, Nakagawa N, Kuramitsu S, Masui R. Protein J 34 411-420 (2015)
  9. Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteria. Mukhopadhyay A, Kladova AV, Bursakov SA, Gavel OY, Calvete JJ, Shnyrov VL, Moura I, Moura JJ, Romão MJ, Trincão J. J Biol Inorg Chem 16 51-61 (2011)
  10. Adenylate Kinase-Catalyzed Reaction of AMP in Pieces: Enzyme Activation for Phosphoryl Transfer to Phosphite Dianion. Fernandez PL, Richard JP. Biochemistry 60 2672-2676 (2021)
  11. Elucidation of Mg²⁺ binding activity of adenylate kinase from Mycobacterium tuberculosis H₃₇Rv using fluorescence studies. Meena LS, Dhakate SR, Sahare PD. Biotechnol Appl Biochem 59 429-436 (2012)
  12. Adenylate Kinase-Catalyzed Reactions of AMP in Pieces: Specificity for Catalysis at the Nucleoside Activator and Dianion Catalytic Sites. Fernandez PL, Richard JP. Biochemistry 61 2766-2775 (2022)
  13. Triple resonance EPR spectroscopy determines the Mn2+ coordination to ATP. Litvinov A, Feintuch A, Un S, Goldfarb D. J Magn Reson 294 143-152 (2018)
  14. Protective efficacy of six recombinant proteins as vaccine candidates against Echinococcus granulosus in dogs. Shao G, Hua R, Song H, Chen Y, Zhu X, Hou W, Li S, Yang A, Yang G. PLoS Negl Trop Dis 17 e0011709 (2023)


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