› 2cf4

X-ray diffraction
3.08Å resolution

Pyrococcus horikoshii TET1 peptidase can assemble into a tetrahedron or a large octahedral shell

Released:
DOI: 10.2210/pdb2cf4/pdb
PDB entry 2cf4 coloured by chain, front view.
PDB entry 2cf4 coloured by chain, side view.
PDB entry 2cf4 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
An archaeal peptidase assembles into two different quaternary structures: A tetrahedron and a giant octahedron.
Schoehn G, Vellieux FM, Asunción Durá M, Receveur-Bréchot V, Fabry CM, Ruigrok RW, Ebel C, Roussel A, Franzetti B
J Biol Chem 281 36327-37 (2006)
PMID: 16973604

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PROTEIN PH0519 Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.21 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58255 (Residues: 1-332; Coverage: 100%)
Gene name: PH0519
Sequence domains: M42 glutamyl aminopeptidase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CO 2 x CO
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: F4132
Unit cell:
a: 221.88Å b: 221.88Å c: 221.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.256 0.332
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Recent advances in the production, properties and applications of haloextremozymes protease and lipase from haloarchaea.
Gaonkar et al. (2023)
18 other articles

7 mentions without citation

Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM.
Heymann JB. (2019)
6 more