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Communication between subunits within an archaeal clamp-loader complex.

Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB
EMBO J 25 2209-18 (2006)
Related entries: 2chq, 2chv

Cited: 23 times
EuropePMC logo PMID: 16628222

Abstract

We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.

Reviews - 2chg mentioned but not cited (1)

  1. Fundamental Characteristics of AAA+ Protein Family Structure and Function. Miller JM, Miller JM, Enemark EJ. Archaea 2016 9294307 (2016)

Articles - 2chg mentioned but not cited (1)

  1. Communication between subunits within an archaeal clamp-loader complex. Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB. EMBO J 25 2209-2218 (2006)


Reviews citing this publication (6)

  1. DNA replication in the archaea. Barry ER, Bell SD. Microbiol Mol Biol Rev 70 876-887 (2006)
  2. The replication clamp-loading machine at work in the three domains of life. Indiani C, O'Donnell M. Nat Rev Mol Cell Biol 7 751-761 (2006)
  3. Replication clamps and clamp loaders. Hedglin M, Kumar R, Benkovic SJ. Cold Spring Harb Perspect Biol 5 a010165 (2013)
  4. AAA+ ATPases in the initiation of DNA replication. Duderstadt KE, Berger JM. Crit Rev Biochem Mol Biol 43 163-187 (2008)
  5. Structural frameworks for considering microbial protein- and nucleic acid-dependent motor ATPases. Thomsen ND, Berger JM. Mol Microbiol 69 1071-1090 (2008)
  6. Processivity factor of DNA polymerase and its expanding role in normal and translesion DNA synthesis. Zhuang Z, Ai Y. Biochim Biophys Acta 1804 1081-1093 (2010)

Articles citing this publication (15)

  1. How a DNA polymerase clamp loader opens a sliding clamp. Kelch BA, Makino DL, O'Donnell M, Kuriyan J. Science 334 1675-1680 (2011)
  2. The 'glutamate switch' provides a link between ATPase activity and ligand binding in AAA+ proteins. Zhang X, Wigley DB. Nat Struct Mol Biol 15 1223-1227 (2008)
  3. The bacterial DnaC helicase loader is a DnaB ring breaker. Arias-Palomo E, O'Shea VL, Hood IV, Berger JM. Cell 153 438-448 (2013)
  4. The replication factor C clamp loader requires arginine finger sensors to drive DNA binding and proliferating cell nuclear antigen loading. Johnson A, Yao NY, Bowman GD, Kuriyan J, O'Donnell M. J Biol Chem 281 35531-35543 (2006)
  5. Conservation of intron and intein insertion sites: implications for life histories of parasitic genetic elements. Swithers KS, Senejani AG, Fournier GP, Gogarten JP. BMC Evol Biol 9 303 (2009)
  6. Mechanism of ATP-driven PCNA clamp loading by S. cerevisiae RFC. Chen S, Levin MK, Sakato M, Zhou Y, Hingorani MM. J Mol Biol 388 431-442 (2009)
  7. Structure of the active form of human origin recognition complex and its ATPase motor module. Tocilj A, On KF, Yuan Z, Sun J, Elkayam E, Li H, Stillman B, Joshua-Tor L. Elife 6 e20818 (2017)
  8. Recognition of the ring-opened state of proliferating cell nuclear antigen by replication factor C promotes eukaryotic clamp-loading. Tainer JA, McCammon JA, Ivanov I. J Am Chem Soc 132 7372-7378 (2010)
  9. Regulation of interactions with sliding clamps during DNA replication and repair. López de Saro FJ. Curr Genomics 10 206-215 (2009)
  10. A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. Sakato M, O'Donnell M, Hingorani MM. J Mol Biol 416 163-175 (2012)
  11. Molecular analyses of a three-subunit euryarchaeal clamp loader complex from Methanosarcina acetivorans. Chen YH, Lin Y, Yoshinaga A, Chhotani B, Lorenzini JL, Crofts AA, Mei S, Mackie RI, Ishino Y, Cann IK. J Bacteriol 191 6539-6549 (2009)
  12. Probing DNA clamps with single-molecule force spectroscopy. Wang L, Xu X, Kumar R, Maiti B, Liu CT, Ivanov I, Lee TH, Benkovic SJ. Nucleic Acids Res 41 7804-7814 (2013)
  13. The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader. Puri N, Fernandez AJ, O'Shea Murray VL, McMillan S, Keck JL, Berger JM. Elife 10 e64232 (2021)
  14. Acipenser iridovirus-European encodes a replication factor C (RFC) sub-unit. Pallandre L, Lesne M, de Boisséson C, Briand FX, Charrier A, Waltzek T, Daniel P, Tragnan A, Debeuf B, Chesneau V, Bigarré L. Arch Virol 163 2985-2995 (2018)
  15. Sulfolobus replication factor C stimulates the activity of DNA polymerase B1. Xing X, Zhang L, Guo L, She Q, Huang L. J Bacteriol 196 2367-2375 (2014)


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