› 2chu

X-ray diffraction
2.4Å resolution

CeuE in complex with mecam

Released:
DOI: 10.2210/pdb2chu/pdb
PDB entry 2chu coloured by chain, front view.
PDB entry 2chu coloured by chain, side view.
PDB entry 2chu coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
An [{Fe(mecam)}2]6- bridge in the crystal structure of a ferric enterobactin binding protein.
Müller A, Wilkinson AJ, Wilson KS, Duhme-Klair AK
Angew Chem Int Ed Engl 45 5132-6 (2006)
PMID: 16927323

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-555487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 32.67 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P8Q4 (Residues: 35-330; Coverage: 96%)
Gene names: Cj1355, ceuE
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ECA 2 x ECA
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P1
Unit cell:
a: 73.072Å b: 42.445Å c: 57.523Å
α: 91.29° β: 97.06° γ: 107.34°
R-values:
R R work R free
0.174 0.17 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Canonical and ECF-type ATP-binding cassette importers in prokaryotes: diversity in modular organization and cellular functions.
Eitinger et al. (2011)
6 more

7 mentions without citation

Characterization of a Bacillus subtilis transporter for petrobactin, an anthrax stealth siderophore.
Zawadzka et al. (2009)
6 more