› 2cju

X-ray diffraction
2.5Å resolution

Crystal structure of the TEPC15-Vk45.1 anti-2-phenyl-5-oxazolone NQ16- 113.8 scFv in complex with phOxGABA

Released:
DOI: 10.2210/pdb2cju/pdb
PDB entry 2cju coloured by chain, front view.
PDB entry 2cju coloured by chain, side view.
PDB entry 2cju coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of affinity maturation of the TEPC15/Vkappa45.1 anti-2-phenyl-5-oxazolone antibodies.
Scotti C, Gherardi E
J Mol Biol 359 1161-9 (2006)
PMID: 16682055

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208526 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig heavy chain V region M603 Chain: H
Molecule details ›
Chain: H
Length: 121 amino acids
Theoretical weight: 13.39 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P01789 (Residues: 1-122; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
NQ16-113.8 ANTI-PHOX ANTIBODY Chain: L
Molecule details ›
Chain: L
Length: 113 amino acids
Theoretical weight: 12.42 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand PHX 1 x PHX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I212121
Unit cell:
a: 51.97Å b: 74.19Å c: 134.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.291
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Isolation and affinity maturation of hapten-specific antibodies.
Sheedy et al. (2007)
3 other articles

4 mentions without citation

Toward high-resolution homology modeling of antibody Fv regions and application to antibody-antigen docking.
Sivasubramanian et al. (2009)
3 more