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Native crystal structure of a nitric oxide-releasing lectin from the seeds of Canavalia maritima.

Gadelha CA, Moreno FB, Santi-Gadelha T, Cajazeiras JB, Rocha BA, Assreuy AM, Lima Mota MR, Pinto NV, Passos Meireles AV, Borges JC, Freitas BT, Canduri F, Souza EP, Delatorre P, Criddle DN, de Azevedo WF, Cavada BS
J Struct Biol 152 185-94 (2005)
Cited: 25 times
EuropePMC logo PMID: 16337811

Abstract

Here, we report the crystallographic study of a lectin from Canavalia maritima seeds (ConM) and its relaxant activity on vascular smooth muscle, to provide new insights into the understanding of structure/function relationships of this class of proteins. ConM was crystallized and its structure determined by standard molecular replacement techniques. The amino acid residues, previously suggested incorrectly by manual sequencing, have now been determined as I17, I53, S129, S134, G144, S164, P165, S187, V190, S169, T196, and S202. Analysis of the structure indicated a dimer in the asymmetric unit, two metal binding sites per monomer, and loops involved in the molecular oligomerization. These confer 98% similarity between ConM and other previously described lectins, derived from Canavalia ensiformis and Canavalia brasiliensis. Our functional data indicate that ConM exerts a concentration-dependent relaxant action on isolated aortic rings that probably occurs via an interaction with a specific lectin-binding site on the endothelium, resulting in a release of nitric oxide.

Reviews - 2cwm mentioned but not cited (1)

  1. ConA-Like Lectins: High Similarity Proteins as Models to Study Structure/Biological Activities Relationships. Cavada BS, Pinto-Junior VR, Osterne VJS, Nascimento KS. Int J Mol Sci 20 E30 (2018)

Articles - 2cwm mentioned but not cited (1)

  1. Effect of lectins from Diocleinae subtribe against oral Streptococci. Cavalcante TT, Anderson Matias da Rocha B, Alves Carneiro V, Vassiliepe Sousa Arruda F, Fernandes do Nascimento AS, Cardoso Sá N, do Nascimento KS, Sousa Cavada B, Holanda Teixeira E. Molecules 16 3530-3543 (2011)


Articles citing this publication (23)

  1. Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules. Delatorre P, Rocha BA, Souza EP, Oliveira TM, Bezerra GA, Moreno FB, Freitas BT, Santi-Gadelha T, Sampaio AH, Azevedo WF, Cavada BS. BMC Struct Biol 7 52 (2007)
  2. Vasodilator effects of Diocleinae lectins from the Canavalia genus. Assreuy AM, Fontenele SR, Pires Ade F, Fernandes DC, Rodrigues NV, Bezerra EH, Moura TR, do Nascimento KS, Cavada BS. Naunyn Schmiedebergs Arch Pharmacol 380 509-521 (2009)
  3. Structural analysis of Canavalia maritima and Canavalia gladiata lectins complexed with different dimannosides: new insights into the understanding of the structure-biological activity relationship in legume lectins. Bezerra GA, Oliveira TM, Moreno FB, de Souza EP, da Rocha BA, Benevides RG, Delatorre P, de Azevedo WF, Cavada BS. J Struct Biol 160 168-176 (2007)
  4. Structural analysis of ConBr reveals molecular correlation between the carbohydrate recognition domain and endothelial NO synthase activation. Bezerra EH, Rocha BA, Nagano CS, Bezerra Gde A, Moura TR, Bezerra MJ, Benevides RG, Sampaio AH, Assreuy AM, Delatorre P, Cavada BS. Biochem Biophys Res Commun 408 566-570 (2011)
  5. Structural studies of an anti-inflammatory lectin from Canavalia boliviana seeds in complex with dimannosides. Bezerra GA, Viertlmayr R, Moura TR, Delatorre P, Rocha BA, do Nascimento KS, Figueiredo JG, Bezerra IG, Teixeira CS, Simões RC, Nagano CS, de Alencar NM, Gruber K, Cavada BS. PLoS One 9 e97015 (2014)
  6. Crystal structure of Dioclea violacea lectin and a comparative study of vasorelaxant properties with Dioclea rostrata lectin. Bezerra MJ, Rodrigues NV, Pires Ade F, Bezerra GA, Nobre CB, Alencar KL, Soares PM, do Nascimento KS, Nagano CS, Martins JL, Gruber K, Sampaio AH, Delatorre P, Rocha BA, Assreuy AM, Cavada BS. Int J Biochem Cell Biol 45 807-815 (2013)
  7. Mass spectrometry and X-ray diffraction analysis of two crystal types of Dioclea virgata lectin: an antinociceptive protein candidate to structure/function analysis. Delatorre P, Rocha BA, Simões RC, Pereira-Júnior FN, Silva HC, Bezerra EH, Bezerra MJ, Marinho ES, Gadelha CA, Santi-Gadelha T, Farias DL, Assreuy AM, Marques-Domingos GF, Nagano CS, Cavada BS. Appl Biochem Biotechnol 164 741-754 (2011)
  8. Potent antiviral activity of carbohydrate-specific algal and leguminous lectins from the Brazilian biodiversity. Gondim ACS, Roberta da Silva S, Mathys L, Noppen S, Liekens S, Holanda Sampaio A, Nagano CS, Renata Costa Rocha C, Nascimento KS, Cavada BS, Sadler PJ, Balzarini J. Medchemcomm 10 390-398 (2019)
  9. Crystal structure of a lectin from Canavalia maritima (ConM) in complex with trehalose and maltose reveals relevant mutation in ConA-like lectins. Delatorre P, Rocha BA, Gadelha CA, Santi-Gadelha T, Cajazeiras JB, Souza EP, Nascimento KS, Freire VN, Sampaio AH, Azevedo WF, Cavada BS. J Struct Biol 154 280-286 (2006)
  10. Purification, partial characterization, and CNBr-sepharose immobilization of a vasorelaxant glucose/mannose lectin from Canavalia virosa seeds. Osterne VJ, Santiago MQ, Pinto-Junior VR, Cajazeiras JB, Correia JL, Leitão CC, Carneiro RF, Pereira-Junior FN, Vasconcelos MA, Rocha BA, Assreuy AM, Bringel PH, Nagano CS, Nascimento KS, Cavada BS. Appl Biochem Biotechnol 172 3342-3353 (2014)
  11. Vasorelaxant activity of Canavalia grandiflora seed lectin: A structural analysis. Barroso-Neto IL, Simões RC, Rocha BA, Bezerra MJ, Pereira-Junior FN, Silva Osterne VJ, Nascimento KS, Nagano CS, Delatorre P, Pereira MG, Freitas Pires A, Sampaio AH, Assreuy AM, Cavada BS. Arch Biochem Biophys 543 31-39 (2014)
  12. Protein crystal content analysis by mass spectrometry and preliminary X-ray diffraction of a lectin from Canavalia grandiflora seeds with modulatory role in inflammation. Simões RC, Rocha BA, Bezerra MJ, Barroso-Neto IL, Pereira-Junior FN, da Mata Moura R, do Nascimento KS, Nagano CS, Delatorre P, de Freitas Pires A, Assreuy AM, Sampaio AH, Cavada BS. Rapid Commun Mass Spectrom 26 811-818 (2012)
  13. Purification and biological activities of Abelmoschus esculentus seed lectin. de Sousa Ferreira Soares G, Assreuy AM, de Almeida Gadelha CA, de Morais Gomes V, Delatorre P, da Conceição Simões R, Cavada BS, Leite JF, Nagano CS, Pinto NV, de Luna Freire Pessoa H, Santi-Gadelha T. Protein J 31 674-680 (2012)
  14. Purification and partial characterization of a novel lectin from Dioclea lasiocarpa Mart seeds with vasodilator effects. do Nascimento AS, Gondim AC, Cajazeiras JB, Correia JL, Pires Ade F, do Nascimento KS, da Silva AL, Nagano CS, Assreuy AM, Cavada BS. J Mol Recognit 25 657-664 (2012)
  15. Purification, characterization and partial sequence of a pro-inflammatory lectin from seeds of Canavalia oxyphylla Standl. & L. O. Williams. Santiago MQ, Leitão CC, Pereira FN, Pinto VR, Osterne VJ, Lossio CF, Cajazeiras JB, Silva HC, Arruda FV, Pereira LP, Assreuy AM, Nascimento KS, Nagano CS, Cavada BS. J Mol Recognit 27 117-123 (2014)
  16. Structural basis of ConM binding with resveratrol, an anti-inflammatory and antioxidant polyphenol. Rocha BA, Teixeira CS, Silva-Filho JC, Nóbrega RB, Alencar DB, Nascimento KS, Freire VN, Gottfried CJ, Nagano CS, Sampaio AH, Saker-Sampaio S, Cavada BS, Delatorre P. Int J Biol Macromol 72 1136-1142 (2015)
  17. Crystal structure of the lectin of Camptosema pedicellatum: implications of a conservative substitution at the hydrophobic subsite. Souza Teixeira C, da Silva HC, de Moura TR, Pereira-Júnior FN, do Nascimento KS, Nagano CS, Sampaio AH, Delatorre P, Rocha BA, Cavada BS. J Biochem 152 87-98 (2012)
  18. Structural analysis of a Dioclea sclerocarpa lectin: Study on the vasorelaxant properties of Dioclea lectins. Barroso-Neto IL, Delatorre P, Teixeira CS, Correia JL, Cajazeiras JB, Pereira RI, Nascimento KS, Laranjeira EP, Pires AF, Assreuy AM, Rocha BA, Cavada BS. Int J Biol Macromol 82 464-470 (2016)
  19. Lectin from Canavalia brasiliensis seeds (ConBr) is a valuable biotechnological tool to stimulate the growth of Rhizobium tropici in vitro. de Vasconcelos MA, Cunha CO, Arruda FV, Carneiro VA, Mercante FM, do Nascimento Neto LG, de Sousa GS, Rocha BA, Teixeira EH, Cavada BS, dos Santos RP. Molecules 17 5244-5254 (2012)
  20. Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity. Pinto-Junior VR, Correia JL, Pereira RI, Pereira-Junior FN, Santiago MQ, Osterne VJ, Madeira JC, Cajazeiras JB, Nagano CS, Delatorre P, Assreuy AM, Nascimento KS, Cavada BS. J Mol Recognit 29 134-141 (2016)
  21. Purification, characterization, and preliminary X-ray diffraction analysis of a lactose-specific lectin from Cymbosema roseum seeds. Rocha BA, Moreno FB, Delatorre P, Souza EP, Marinho ES, Benevides RG, Rustiguel JK, Souza LA, Nagano CS, Debray H, Sampaio AH, de Azevedo WF, Cavada BS. Appl Biochem Biotechnol 152 383-393 (2009)
  22. CRLI induces vascular smooth muscle relaxation and suggests a dual mechanism of eNOS activation by legume lectins via muscarinic receptors and shear stress. Rocha BA, Barroso-Neto IL, Teixeira CS, Santiago MQ, Pires AF, Souza LA, Nascimento KS, Sampaio AH, Delatorre P, Assreuy AM, Cavada BS. Arch Biochem Biophys 565 32-39 (2015)
  23. Canarosine: a new guanidine alkaloid from Canavalia rosea with inhibitory activity on dopamine D1 receptors. Pattamadilok D, Pengsuparp T, Phummiratch D, Ongpipattanakul B, Meksuriyen D, Kawanishi K, Kaneda N, Suttisri R. J Asian Nat Prod Res 10 915-918 (2008)


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