› 2d6p

X-ray diffraction
2.7Å resolution

Crystal structure of mouse galectin-9 N-terminal CRD in complex with T-antigen

Released:
DOI: 10.2210/pdb2d6p/pdb
PDB entry 2d6p coloured by chain, front view.
PDB entry 2d6p coloured by chain, side view.
PDB entry 2d6p coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the galectin-9 N-terminal carbohydrate recognition domain from Mus musculus reveals the basic mechanism of carbohydrate recognition.
Nagae M, Nishi N, Murata T, Usui T, Nakamura T, Wakatsuki S, Kato R
J Biol Chem 281 35884-93 (2006)
PMID: 16990264

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-517656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.17 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O08573 (Residues: 1-147; Coverage: 42%)
  • Best match: O08573-2 (Residues: 1-157)
Gene name: Lgals9
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NGA, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P41212
Unit cell:
a: 58.051Å b: 58.051Å c: 222.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Galectins: structure, function and therapeutic potential.
Yang et al. (2008)
15 more

1 mention without citation

A combination of biochemical and proteomic analyses reveals Bx-LEC-1 as an antigenic target for the monoclonal antibody 3-2A7-2H5-D9-F10 specific to the pine wood nematode.
Lee et al. (2011)