› 2de6

X-ray diffraction
1.8Å resolution

The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase

Released:
DOI: 10.2210/pdb2de6/pdb
PDB entry 2de6 coloured by chain, front view.
PDB entry 2de6 coloured by chain, side view.
PDB entry 2de6 coloured by chain, top view.
Source organisms:
Primary publication:
Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system.
Ashikawa Y, Fujimoto Z, Noguchi H, Habe H, Omori T, Yamane H, Nojiri H
Structure 14 1779-89 (2006)
PMID: 17161368

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183053 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rieske domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 392 amino acids
Theoretical weight: 44.91 KDa
Source organism: Janthinobacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84II6 (Residues: 1-384; Coverage: 100%)
Gene name: carAa
Sequence domains:
Structure domains:
Ferredoxin CarAc Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 115 amino acids
Theoretical weight: 12.45 KDa
Source organism: Pseudomonas resinovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GI16 (Residues: 1-107; Coverage: 100%)
Gene name: carAc
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

2 bound ligands:
Ligand FE2 3 x FE2
Ligand FES 6 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 98.225Å b: 90.012Å c: 105.523Å
α: 90° β: 104.14° γ: 90°
R-values:
R R work R free
0.187 0.187 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Mononuclear non-heme iron enzymes with the 2-His-1-carboxylate facial triad: recent developments in enzymology and modeling studies.
Bruijnincx et al. (2008)
4 more

5 mentions without citation

Prohibitin Interacts with envelope proteins of white spot syndrome virus and prevents infection in the red swamp crayfish, Procambarus clarkii.
Lan et al. (2013)
4 more