Function and Biology Details
Reaction catalysed:
A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
tRNA-specific 2-thiouridylase MnmA and RNA (preferred)
PDBe Complex ID:
PDB-CPX-150571 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA-specific 2-thiouridylase MnmA
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 42.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 380 amino acids
Theoretical weight: 42.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical:
P25745 (Residues: 1-368; Coverage: 100%)
Sequence domains:
- tRNA methyl transferase HUP domain
- tRNA methyl transferase PRC-barrel domain
- Aminomethyltransferase beta-barrel domain
