› 2e5a

X-ray diffraction
2.1Å resolution

Crystal Structure of Bovine Lipoyltransferase in Complex with Lipoyl-AMP

Released:
DOI: 10.2210/pdb2e5a/pdb
PDB entry 2e5a coloured by chain, front view.
PDB entry 2e5a coloured by chain, side view.
PDB entry 2e5a coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of bovine lipoyltransferase in complex with lipoyl-AMP.
Fujiwara K, Hosaka H, Matsuda M, Okamura-Ikeda K, Motokawa Y, Suzuki M, Nakagawa A, Taniguchi H
J Mol Biol 371 222-34 (2007)
PMID: 17570395

Function and Biology Details

Reaction catalysed: 
[Glycine cleavage system H]-N(6)-lipoyl-L-lysine + a [lipoyl-carrier protein] = [glycine cleavage system H] + a [lipoyl-carrier protein]-N(6)-lipoyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-520299 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoyl amidotransferase LIPT1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 39.21 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: O46419 (Residues: 27-373; Coverage: 93%)
Gene name: LIPT1
Sequence domains: Lipoyl protein ligase A/B catalytic domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand PO4 2 x PO4
Ligand LAQ 1 x LAQ
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 81.439Å b: 81.439Å c: 120.532Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.219 0.263
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Mitochondrial fatty acid synthesis type II: more than just fatty acids.
Hiltunen et al. (2009)
7 more

5 mentions without citation

Functional Assessment of Lipoyltransferase-1 Deficiency in Cells, Mice, and Humans.
Ni et al. (2019)
4 more