› 2eak

X-ray diffraction
1.97Å resolution

Crystal structure of human galectin-9 N-terminal CRD in complex with lactose

Released:
DOI: 10.2210/pdb2eak/pdb
PDB entry 2eak coloured by chain, front view.
PDB entry 2eak coloured by chain, side view.
PDB entry 2eak coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the human galectin-9 N-terminal carbohydrate recognition domain reveals unexpected properties that differ from the mouse orthologue.
Nagae M, Nishi N, Nakamura-Tsuruta S, Hirabayashi J, Wakatsuki S, Kato R
J Mol Biol 375 119-35 (2008)
PMID: 18005988

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-9 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 148 amino acids
Theoretical weight: 16.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00182 (Residues: 1-148; Coverage: 42%)
Gene name: LGALS9
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL
Carbohydrate polymer
2 bound ligands:
Ligand DTV 1 x DTV
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 70.681Å b: 118.687Å c: 132.052Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Therapeutic potential of Galectin-9 in human disease.
Wiersma et al. (2013)
7 more

3 mentions without citation

New targets for HIV drug discovery.
Puhl et al. (2019)
2 more