› 2ecq

X-ray diffraction
1.9Å resolution

Crystal Structure of T.th. HB8 O-acetylserine sulfhydrylase Complexed with 3-Hydroxylactate

Released:
DOI: 10.2210/pdb2ecq/pdb
PDB entry 2ecq coloured by chain, front view.
PDB entry 2ecq coloured by chain, side view.
PDB entry 2ecq coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry author: Goto M

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-562377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 33.07 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLE6 (Residues: 1-304; Coverage: 100%)
Gene name: TTHA0347
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand 3HL 1 x 3HL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 104.531Å b: 104.531Å c: 79.802Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.179 0.2
Expression system: Escherichia coli

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