› 2el9

X-ray diffraction
2.7Å resolution

Crystal structure of E.coli Histidyl-tRNA synthetase complexed with a histidyl-adenylate analogue

Released:
DOI: 10.2210/pdb2el9/pdb
PDB entry 2el9 coloured by chain, front view.
PDB entry 2el9 coloured by chain, side view.
PDB entry 2el9 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Yanagisawa T, Si SY, Matsuno M, Ishii R, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-548473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine--tRNA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60906 (Residues: 1-424; Coverage: 100%)
Gene names: JW2498, b2514, hisS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HSS 4 x HSS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 60.113Å b: 108.345Å c: 193.645Å
α: 90° β: 98.21° γ: 90°
R-values:
R R work R free
0.227 0.227 0.285
Expression system: Escherichia coli BL21(DE3)

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