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C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity.

Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K
J Biol Chem 284 29625-34 (2009)
Related entries: 2eqq, 2eqt

Cited: 3 times
EuropePMC logo PMID: 19710009

Abstract

Growth-blocking peptide (GBP) is a hormone-like peptide that suppresses the growth of the host armyworm. Although the 23-amino acid GBP (1-23 GBP) is expressed in nonparasitized armyworm plasma, the parasitization by wasp produces the 28-amino acid GBP (1-28 GBP) through an elongation of the C-terminal amino acid sequence. In this study, we characterized the GBP variants, which consist of various lengths of the C-terminal region, by comparing their biological activities and three-dimensional structures. The results of an injection study indicate that 1-28 GBP most strongly suppresses larval growth. NMR analysis shows that these peptides have basically the same tertiary structures and that the extension of the C-terminal region is disordered. However, the C-terminal region of 1-28 GBP undergoes a conformational transition from a random coiled state to an alpha-helical state in the presence of dodecylphosphocholine micelles. This suggests that binding of the C-terminal region would affect larval growth activity.

Articles - 2eqh mentioned but not cited (1)

  1. C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity. Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K. J Biol Chem 284 29625-29634 (2009)


Articles citing this publication (2)

  1. Sea anemone peptide with uncommon β-hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity. Osmakov DI, Kozlov SA, Andreev YA, Koshelev SG, Sanamyan NP, Sanamyan KE, Dyachenko IA, Bondarenko DA, Murashev AN, Mineev KS, Arseniev AS, Grishin EV. J Biol Chem 288 23116-23127 (2013)
  2. Letter Biophysical research in Hokkaido University, Japan. Aizawa T, Demura M, Gohara K, Haga H, Ishimori K, Kinjo M, Komatsuzaki T, Maenaka K, Yao M. Biophys Rev 12 233-236 (2020)


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