› 2ev1

X-ray diffraction
1.6Å resolution

Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0

Released:
DOI: 10.2210/pdb2ev1/pdb
PDB entry 2ev1 coloured by chain, front view.
PDB entry 2ev1 coloured by chain, side view.
PDB entry 2ev1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid.
Findeisen F, Linder JU, Schultz A, Schultz JE, Brügger B, Wieland F, Sinning I, Tews I
J Mol Biol 369 1282-95 (2007)
PMID: 17482646

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pH-sensitive adenylate cyclase Rv1264 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24.17 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMU9 (Residues: 1-207; Coverage: 52%)
Gene names: MTCY50.18c, Rv1264
Sequence domains: Adenylate cyclase regulatory domain

Ligands and Environments

2 bound ligands:
Ligand OLA 2 x OLA
Ligand 1PE 2 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 86.6Å b: 93.528Å c: 54.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress.
Hentchel et al. (2015)
4 more