2exy

X-ray diffraction
3.1Å resolution

Crystal structure of the E148Q Mutant of EcClC, Fab complexed in absence of bound ions

Released:
Model geometry
Fit model/data
Source organisms:
Primary publication:
Ion-binding properties of the ClC chloride selectivity filter.
EMBO J 25 24-33 (2006)
PMID: 16341087

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208528 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 50.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Ig heavy chain V region T601 Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01808 (Residues: 1-101, 102-104, 105-118; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Fab Fragment (Light Chain) Chains: D, F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 219.64Å b: 119.788Å c: 151.3Å
α: 90° β: 128.09° γ: 90°
R-values:
R R work R free
0.328 0.328 0.352
Expression system: Escherichia coli BL21(DE3)