› 2f24

X-ray diffraction
1.76Å resolution

Crystal Structure of the Human Sialidase Neu2 E111Q Mutant

Released:
DOI: 10.2210/pdb2f24/pdb
PDB entry 2f24 coloured by chain, front view.
PDB entry 2f24 coloured by chain, side view.
PDB entry 2f24 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Chavas LMG, Kato R, Fusi P, Tringali C, Venerando B, Tettamanti G, Monti E, Wakatsuki S

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase-2 Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 42.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Y3R4 (Residues: 1-380; Coverage: 100%)
Gene name: NEU2
Sequence domains: BNR repeat-like domain
Structure domains: Neuraminidase

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: R3
Unit cell:
a: 145.52Å b: 145.52Å c: 64.08Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.217
Expression system: Escherichia coli BL21

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