› 2f6f

X-ray diffraction
2Å resolution

The structure of the S295F mutant of human PTP1B

Released:
DOI: 10.2210/pdb2f6f/pdb
PDB entry 2f6f coloured by chain, front view.
PDB entry 2f6f coloured by chain, side view.
PDB entry 2f6f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Residues distant from the active site influence protein-tyrosine phosphatase 1B inhibitor binding.
Montalibet J, Skorey K, McKay D, Scapin G, Asante-Appiah E, Kennedy BP
J Biol Chem 281 5258-66 (2006)
PMID: 16332678

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 35.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18031 (Residues: 1-298; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P3121
Unit cell:
a: 88.33Å b: 88.33Å c: 104.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.183 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Inside the human cancer tyrosine phosphatome.
Julien et al. (2011)
4 more

6 mentions without citation

Structural analysis of cysteine S-nitrosylation: a modified acid-based motif and the emerging role of trans-nitrosylation.
Marino et al. (2010)
5 more