› 2fnk

X-ray diffraction
1.8Å resolution

Activation of Human Carbonic Anhydrase II by exogenous proton donors

Released:
DOI: 10.2210/pdb2fnk/pdb
PDB entry 2fnk coloured by chain, front view.
PDB entry 2fnk coloured by chain, side view.
PDB entry 2fnk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Location of binding sites in small molecule rescue of human carbonic anhydrase II.
Bhatt D, Fisher SZ, Tu C, McKenna R, Silverman DN
Biophys J 92 562-70 (2007)
PMID: 17071654

Function and Biology Details

Reactions catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Urea = cyanamide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00918 (Residues: 1-260; Coverage: 100%)
Gene name: CA2
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 42.092Å b: 41.402Å c: 71.928Å
α: 90° β: 104.005° γ: 90°
R-values:
R R work R free
0.204 0.204 0.242
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding.
Krishnamurthy et al. (2008)
1 more

1 mention without citation

Location of binding sites in small molecule rescue of human carbonic anhydrase II.
Bhatt et al. (2007)