› 2fr4

X-ray diffraction
1.95Å resolution

Structure of Fab DNA-1 complexed with a stem-loop DNA ligand

Released:
DOI: 10.2210/pdb2fr4/pdb
PDB entry 2fr4 coloured by chain, front view.
PDB entry 2fr4 coloured by chain, side view.
PDB entry 2fr4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Impact of DNA hairpin folding energetics on antibody-ssDNA association.
Ou Z, Bottoms CA, Henzl MT, Tanner JJ
J Mol Biol 374 1029-40 (2007)
PMID: 18028946

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-207783 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
antibody light chain FAB Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody heavy chain FAB Chains: B, H
Molecule details ›
Chains: B, H
Length: 230 amino acids
Theoretical weight: 24.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
5'-D(*CP*TP*GP*CP*CP*TP*TP*CP*AP*G)-3' Chains: M, N
Molecule details ›
Chains: M, N
Length: 10 nucleotides
Theoretical weight: 3 KDa

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 84.757Å b: 90.45Å c: 128.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.256
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A survey of the year 2007 literature on applications of isothermal titration calorimetry.
Bjelić et al. (2008)
5 other articles

2 mentions without citation

Impact of DNA hairpin folding energetics on antibody-ssDNA association.
Ou et al. (2007)
1 more