› 2fu3

X-ray diffraction
2.7Å resolution

Crystal structure of gephyrin E-domain

Released:
DOI: 10.2210/pdb2fu3/pdb
PDB entry 2fu3 coloured by chain, front view.
PDB entry 2fu3 coloured by chain, side view.
PDB entry 2fu3 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Deciphering the structural framework of glycine receptor anchoring by gephyrin.
Kim EY, Schrader N, Smolinsky B, Bedet C, Vannier C, Schwarz G, Schindelin H
EMBO J 25 1385-95 (2006)
PMID: 16511563

Function and Biology Details

Reactions catalysed: 
Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gephyrin Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 45.65 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03555 (Residues: 350-768; Coverage: 55%)
Gene names: Gph, Gphn
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21212
Unit cell:
a: 108.82Å b: 156.195Å c: 51.362Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Gephyrin: a master regulator of neuronal function?
Tyagarajan et al. (2014)
19 more

4 mentions without citation

Protein Dynamics in F-like Bacterial Conjugation.
Bragagnolo et al. (2020)
3 more