› 2fub

X-ray diffraction
2.3Å resolution

Crystal structure of urate oxidase at 140 MPa

Released:
DOI: 10.2210/pdb2fub/pdb
PDB entry 2fub coloured by chain, front view.
PDB entry 2fub coloured by chain, side view.
PDB entry 2fub coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
High pressure macromolecular crystallography: the 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly.
Colloc'h N, Girard E, Dhaussy AC, Kahn R, Ascone I, Mezouar M, Fourme R
Biochim Biophys Acta 1764 391-7 (2006)
PMID: 16478683

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.16 KDa
Source organism: Aspergillus flavus
UniProt:
  • Canonical: Q00511 (Residues: 2-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

2 bound ligands:
Ligand AZA 1 x AZA
Ligand CYS 1 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: I222
Unit cell:
a: 79.18Å b: 95.63Å c: 104.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.213

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Citations

2 review citations

High-pressure protein crystallography and NMR to explore protein conformations.
Collins et al. (2011)
1 more