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Crystal structures of human cardiac beta-myosin II S2-Delta provide insight into the functional role of the S2 subfragment.

Blankenfeldt W, Thomä NH, Wray JS, Gautel M, Schlichting I
Proc Natl Acad Sci U S A 103 17713-7 (2006)
Related entries: 1nkn, 2fxm

Cited: 72 times
EuropePMC logo PMID: 17095604

Abstract

Myosin II is the major component of the muscle thick filament. It consists of two N-terminal S1 subfragments ("heads") connected to a long dimeric coiled-coil rod. The rod is in itself twofold symmetric, but in the filament, the two heads point away from the filament surface and are therefore not equivalent. This breaking of symmetry requires the initial section of the rod, subfragment 2 (S2), to be relatively flexible. S2 is an important functional element, involved in various mechanisms by which the activity of smooth and striated muscle is regulated. We have determined crystal structures of the 126 N-terminal residues of S2 from human cardiac beta-myosin II (S2-Delta), of both WT and the disease-associated E924K mutant. S2-Delta is a straight parallel dimeric coiled coil, but the N terminus of one chain is disordered in WT-S2-Delta due to crystal contacts, indicative of unstable local structure. Bulky noncanonical side chains pack into a/d positions of S2-Delta's N terminus, leading to defined local asymmetry and axial stagger, which could induce nonequivalence of the S1 subfragments. Additionally, S2 possesses a conserved charge distribution with three prominent rings of negative potential within S2-Delta, the first of which may provide a binding interface for the "blocked head" of smooth muscle myosin in the OFF state. The observation that many disease-associated mutations affect the second negatively charged ring further suggests that charge interactions play an important role in regulation of cardiac muscle activity through myosin-binding protein C.

Articles - 2fxo mentioned but not cited (10)

  1. Crystal structures of human cardiac beta-myosin II S2-Delta provide insight into the functional role of the S2 subfragment. Blankenfeldt W, Thomä NH, Wray JS, Gautel M, Schlichting I. Proc Natl Acad Sci U S A 103 17713-17717 (2006)
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Reviews citing this publication (13)

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Articles citing this publication (49)

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  5. Structure of an integrin alphaIIb beta3 transmembrane-cytoplasmic heterocomplex provides insight into integrin activation. Yang J, Ma YQ, Page RC, Misra S, Plow EF, Qin J. Proc Natl Acad Sci U S A 106 17729-17734 (2009)
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Related citations provided by authors (2)

  1. Mutations in beta-myosin S2 that cause familial hypertrophic cardiomyopathy (FHC) abolish the interaction with the regulatory domain of myosin-binding protein-C.. Gruen M, Gautel M J Mol Biol 286 933-49 (1999)
  2. Visualization of an unstable coiled coil from the scallop myosin rod.. Li Y, Brown JH, Reshetnikova L, Blazsek A, Farkas L, Nyitray L, Cohen C Nature 424 341-5 (2003)

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