› 2g6x

X-ray diffraction
2Å resolution

Crystal structure of a novel green fluorescent protein from marine copepod Pontellina plumata

Released:
DOI: 10.2210/pdb2g6x/pdb
PDB entry 2g6x coloured by chain, front view.
PDB entry 2g6x coloured by chain, side view.
PDB entry 2g6x coloured by chain, top view.
Source organism: Pontellina plumata
Primary publication:
Structural basis for the fast maturation of Arthropoda green fluorescent protein.
Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM
EMBO Rep 7 1006-12 (2006)
PMID: 16936637

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 217 amino acids
Theoretical weight: 24.39 KDa
Source organism: Pontellina plumata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WV12 (Residues: 2-219; Coverage: 98%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 4 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 97.747Å b: 97.91Å c: 172.316Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.246
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
14 more

2 mentions without citation

Structural basis for the fast maturation of Arthropoda green fluorescent protein.
Evdokimov et al. (2006)
1 more