› 2ga3

X-ray diffraction
2.2Å resolution

Structure of S102T E. coli Alkaline Phosphatase-phosphate intermediate at 2.20A resolution

Released:
DOI: 10.2210/pdb2ga3/pdb
PDB entry 2ga3 coloured by chain, front view.
PDB entry 2ga3 coloured by chain, side view.
PDB entry 2ga3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Trapping the tetrahedral intermediate in the alkaline phosphatase reaction by substitution of the active site serine with threonine.
Wang J, Kantrowitz ER
Protein Sci 15 2395-401 (2006)
PMID: 17008720

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 47.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 23-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 2 x MG
Ligand SO4 2 x SO4
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 76.23Å b: 164.97Å c: 192.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.229
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

High-resolution analysis of Zn(2+) coordination in the alkaline phosphatase superfamily by EXAFS and x-ray crystallography.
Bobyr et al. (2012)
6 more

3 mentions without citation

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
2 more