› 2gdg

X-ray diffraction
1.45Å resolution

Crystal structure of covalently modified macrophage inhibitory factor

Released:
DOI: 10.2210/pdb2gdg/pdb
PDB entry 2gdg coloured by chain, front view.
PDB entry 2gdg coloured by chain, side view.
PDB entry 2gdg coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Inactivation of the phenylpyruvate tautomerase activity of macrophage migration inhibitory factor by 2-oxo-4-phenyl-3-butynoate.
Golubkov PA, Johnson WH, Czerwinski RM, Person MD, Wang SC, Whitman CP, Hackert ML
Bioorg Chem 34 183-99 (2006)
PMID: 16780921

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-543149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P34884 (Residues: 2-115; Coverage: 99%)
Gene name: Mif
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P63
Unit cell:
a: 94.705Å b: 94.705Å c: 87.674Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.147 0.183
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Targeting MIF in Cancer: Therapeutic Strategies, Current Developments, and Future Opportunities.
O'Reilly et al. (2016)
1 more

3 mentions without citation

The conformational flexibility of the carboxy terminal residues 105-114 is a key modulator of the catalytic activity and stability of macrophage migration inhibitory factor.
El-Turk et al. (2008)
2 more