› 2gen

X-ray diffraction
1.7Å resolution

Structural Genomics, the crystal structure of a probable transcriptional regulator from Pseudomonas aeruginosa PAO1

Released:
DOI: 10.2210/pdb2gen/pdb
PDB entry 2gen coloured by chain, front view.
PDB entry 2gen coloured by chain, side view.
PDB entry 2gen coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Tan K, Skarina T, Onopriyenko O, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 21.83 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I2Q9 (Residues: 1-193; Coverage: 100%)
Gene name: PA1836
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 38.335Å b: 69.13Å c: 176.864Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.248
Expression system: Escherichia coli BL21

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