› 2gg9

X-ray diffraction
1.05Å resolution

Novel bacterial methionine aminopeptidase inhibitors

Released:
DOI: 10.2210/pdb2gg9/pdb
PDB entry 2gg9 coloured by chain, front view.
PDB entry 2gg9 coloured by chain, side view.
PDB entry 2gg9 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Serendipitous discovery of novel bacterial methionine aminopeptidase inhibitors.
Evdokimov AG, Pokross M, Walter RL, Mekel M, Barnett BL, Amburgey J, Seibel WL, Soper SJ, Djung JF, Fairweather N, Diven C, Rastogi V, Grinius L, Klanke C, Siehnel R, Twinem T, Andrews R, Curnow A
Proteins 66 538-46 (2007)
PMID: 17120228

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AE18 (Residues: 2-264; Coverage: 100%)
Gene names: JW0163, b0168, map
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

3 bound ligands:
Ligand CO 2 x CO
Ligand NA 1 x NA
Ligand U16 1 x U16
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 39.131Å b: 63.29Å c: 52.513Å
α: 90° β: 109.71° γ: 90°
R-values:
R R work R free
0.141 0.14 0.159
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Challenges of antibacterial discovery.
Silver LL. (2011)
1 more

1 mention without citation

Advances in Bacterial Methionine Aminopeptidase Inhibition.
Helgren et al. (2016)