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The structure of the human centrin 2-xeroderma pigmentosum group C protein complex.

Thompson JR, Ryan ZC, Salisbury JL, Kumar R
J Biol Chem 281 18746-52 (2006)
Cited: 68 times
EuropePMC logo PMID: 16627479

Abstract

Human centrin-2 plays a key role in centrosome function and stimulates nucleotide excision repair by binding to the xeroderma pigmentosum group C protein. To determine the structure of human centrin-2 and to develop an understanding of molecular interactions between centrin and xeroderma pigmentosum group C protein, we characterized the crystal structure of calcium-loaded full-length centrin-2 complexed with a xeroderma pigmentosum group C peptide. Our structure shows that the carboxyl-terminal domain of centrin-2 binds this peptide and two calcium atoms, whereas the amino-terminal lobe is in a closed conformation positioned distantly by an ordered alpha-helical linker. A stretch of the amino-terminal domain unique to centrins appears disordered. Two xeroderma pigmentosum group C peptides both bound to centrin-2 also interact to form an alpha-helical coiled-coil. The interface between centrin-2 and each peptide is predominantly nonpolar, and key hydrophobic residues of XPC have been identified that lead us to propose a novel binding motif for centrin.

Reviews - 2ggm mentioned but not cited (4)

  1. DNA repair gets physical: mapping an XPA-binding site on ERCC1. Croteau DL, Peng Y, Van Houten B. DNA Repair (Amst) 7 819-826 (2008)
  2. Advances in imaging of understudied ions in signaling: A focus on magnesium. Lazarou TS, Buccella D. Curr Opin Chem Biol 57 27-33 (2020)
  3. Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition. Pedretti M, Bombardi L, Conter C, Favretto F, Dominici P, Astegno A. Int J Mol Sci 22 12173 (2021)
  4. Every protagonist has a sidekick: Structural aspects of human xeroderma pigmentosum-binding proteins in nucleotide excision repair. Feltes BC. Protein Sci 30 2187-2205 (2021)

Articles - 2ggm mentioned but not cited (15)



Reviews citing this publication (7)

  1. Molecular mechanisms of DNA damage recognition for mammalian nucleotide excision repair. Sugasawa K. DNA Repair (Amst) 44 110-117 (2016)
  2. Centrins in retinal photoreceptor cells: regulators in the connecting cilium. Trojan P, Krauss N, Choe HW, Giessl A, Pulvermüller A, Wolfrum U. Prog Retin Eye Res 27 237-259 (2008)
  3. A mechanistic view on the evolutionary origin for centrin-based control of centriole duplication. Salisbury JL. J Cell Physiol 213 420-428 (2007)
  4. Such small hands: the roles of centrins/caltractins in the centriole and in genome maintenance. Dantas TJ, Daly OM, Morrison CG. Cell Mol Life Sci 69 2979-2997 (2012)
  5. Overview of xeroderma pigmentosum proteins architecture, mutations and post-translational modifications. Feltes BC, Bonatto D. Mutat Res Rev Mutat Res 763 306-320 (2015)
  6. Centrins in unicellular organisms: functional diversity and specialization. Zhang Y, He CY. Protoplasma 249 459-467 (2012)
  7. Insights into functional aspects of centrins from the structure of N-terminally extended mouse centrin 1. Park JH, Pulvermüller A, Scheerer P, Rausch S, Giessl A, Höhne W, Wolfrum U, Hofmann KP, Ernst OP, Choe HW, Krauss N. Vision Res 46 4568-4574 (2006)

Articles citing this publication (42)



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