2gje

X-ray diffraction
3.37Å resolution

Structure of a guideRNA-binding protein complex bound to a gRNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-161039 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (4 distinct):
SUN domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.06 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q952G2 (Residues: 30-224; Coverage: 87%)
Sequence domains: Mitochondrial RNA binding protein MRP
Structure domains: Transcriptional Co-activator pc4; Chain A
SUN domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 187 amino acids
Theoretical weight: 21.33 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P90629 (Residues: 20-206; Coverage: 91%)
Gene name: gBP21
Sequence domains: Mitochondrial RNA binding protein MRP
Structure domains: Transcriptional Co-activator pc4; Chain A
guide RNA 40-mer Chain: R
Molecule details ›
Chain: R
Length: 39 nucleotides
Theoretical weight: 12.53 KDa
RNA tetramer Chain: S
Molecule details ›
Chain: S
Length: 5 nucleotides
Theoretical weight: 1.59 KDa

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6122
Unit cell:
a: 157.6Å b: 157.6Å c: 81.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.282 0.282 0.3
Expression system: Escherichia coli BL21(DE3)