› 2gjl

X-ray diffraction
2Å resolution

Crystal Structure of 2-nitropropane dioxygenase

Released:
DOI: 10.2210/pdb2gjl/pdb
PDB entry 2gjl coloured by chain, front view.
PDB entry 2gjl coloured by chain, side view.
PDB entry 2gjl coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base.
Ha JY, Min JY, Lee SK, Kim HS, Kim DJ, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW
J Biol Chem 281 18660-7 (2006)
PMID: 16682407

Function and Biology Details

Reaction catalysed: 
NADH + a quinone = NAD(+) + a quinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-191386 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:quinone reductase Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 34.86 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I4V0 (Residues: 1-328; Coverage: 100%)
Gene name: PA1024
Sequence domains: Nitronate monooxygenase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A, PAL/PLS BEAMLINE 6B
Spacegroup: P3121
Unit cell:
a: 93.546Å b: 93.546Å c: 80.704Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.227
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Flavin dependent monooxygenases.
Huijbers et al. (2014)
2 more

7 mentions without citation

Crystal structure of enoyl-acyl carrier protein reductase (FabK) from Streptococcus pneumoniae reveals the binding mode of an inhibitor.
Saito et al. (2008)
6 more