› 2gmn

X-ray diffraction
1.4Å resolution

Crystal structure of BJP-1, a subclass B3 metallo-beta-lactamase of Bradyrhizobium japonicum

Released:
DOI: 10.2210/pdb2gmn/pdb
PDB entry 2gmn coloured by chain, front view.
PDB entry 2gmn coloured by chain, side view.
PDB entry 2gmn coloured by chain, top view.
Source organism: Bradyrhizobium diazoefficiens USDA 110
Primary publication:
Postgenomic scan of metallo-beta-lactamase homologues in rhizobacteria: identification and characterization of BJP-1, a subclass B3 ortholog from Bradyrhizobium japonicum.
Stoczko M, Frère JM, Rossolini GM, Docquier JD
Antimicrob Agents Chemother 50 1973-81 (2006)
PMID: 16723554

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 29.94 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q89GW5 (Residues: 21-294; Coverage: 100%)
Gene name: blr6230
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 42.501Å b: 44.773Å c: 76.966Å
α: 78.92° β: 89.51° γ: 61.91°
R-values:
R R work R free
0.18 0.176 0.213
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Metallo-beta-lactamases (classification, activity, genetic organization, structure, zinc coordination) and their superfamily.
Bebrone C. (2007)
7 more

4 mentions without citation

Crystal structure of the mobile metallo-β-lactamase AIM-1 from Pseudomonas aeruginosa: insights into antibiotic binding and the role of Gln157.
Leiros et al. (2012)
3 more