› 2gp6

X-ray diffraction
2.4Å resolution

X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB)

Released:
DOI: 10.2210/pdb2gp6/pdb
PDB entry 2gp6 coloured by chain, front view.
PDB entry 2gp6 coloured by chain, side view.
PDB entry 2gp6 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB).
Sridharan S, Wang L, Brown AK, Dover LG, Kremer L, Besra GS, Sacchettini JC
J Mol Biol 366 469-80 (2007)
PMID: 17174327

Function and Biology Details

Reaction catalysed: 
An ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO(2) + a holo-[acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 434 amino acids
Theoretical weight: 46.15 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQD7 (Residues: 5-417; Coverage: 99%)
Gene names: MTCY427.27, Rv2246, kasB
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: R3
Unit cell:
a: 198.682Å b: 198.682Å c: 71.831Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Mycobacterium tuberculosis FAS-II condensing enzymes: their role in mycolic acid biosynthesis, acid-fastness, pathogenesis and in future drug development.
Bhatt et al. (2007)
4 more

8 mentions without citation

Structural classification and properties of ketoacyl synthases.
Chen et al. (2011)
7 more