› 2grz

X-ray diffraction
1.6Å resolution

5ns Photoproduct of the M37V mutant of Scapharca HbI

Released:
DOI: 10.2210/pdb2grz/pdb
PDB entry 2grz coloured by chain, ensemble of 2 models, front view.
PDB entry 2grz coloured by chain, ensemble of 2 models, side view.
PDB entry 2grz coloured by chain, ensemble of 2 models, top view.
Source organism: Anadara inaequivalvis
Primary publication:
Allosteric action in real time: time-resolved crystallographic studies of a cooperative dimeric hemoglobin.
Knapp JE, Pahl R, Srajer V, Royer WE
Proc Natl Acad Sci U S A 103 7649-54 (2006)
PMID: 16684887

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 15.94 KDa
Source organism: Anadara inaequivalvis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02213 (Residues: 1-146; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand CMO 2 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2
Unit cell:
a: 93.04Å b: 43.93Å c: 83.44Å
α: 90° β: 121.95° γ: 90°
R-values:
R R work R free
0.106 0.106 0.109
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Allostery and cooperativity revisited.
Cui et al. (2008)
9 more

4 mentions without citation

Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering.
Yang et al. (2018)
3 more