› 2h46

X-ray diffraction
1.9Å resolution

Native domain-swapped dimer crystal structure of the Grb2 SH2 domain

Released:
DOI: 10.2210/pdb2h46/pdb
PDB entry 2h46 coloured by chain, front view.
PDB entry 2h46 coloured by chain, side view.
PDB entry 2h46 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and energetic aspects of Grb2-SH2 domain-swapping.
Benfield AP, Whiddon BB, Clements JH, Martin SF
Arch Biochem Biophys 462 47-53 (2007)
PMID: 17466257

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-158872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Growth factor receptor-bound protein 2 Chain: E
Molecule details ›
Chain: E
Length: 116 amino acids
Theoretical weight: 13.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62993 (Residues: 53-162; Coverage: 51%)
Gene names: ASH, GRB2
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I422
Unit cell:
a: 80.783Å b: 80.783Å c: 75.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.222 0.231
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The language of SH2 domain interactions defines phosphotyrosine-mediated signal transduction.
Liu et al. (2012)
5 more

2 mentions without citation

Structural and energetic aspects of Grb2-SH2 domain-swapping.
Benfield et al. (2007)
1 more