› 2hgh

Solution NMR

Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer (NMR structure)

Released:
DOI: 10.2210/pdb2hgh/pdb
PDB entry 2hgh coloured by chain, ensemble of 20 models, front view.
PDB entry 2hgh coloured by chain, ensemble of 20 models, side view.
PDB entry 2hgh coloured by chain, ensemble of 20 models, top view.
Source organism: Xenopus laevis
Primary publication:
Induced fit and "lock and key" recognition of 5S RNA by zinc fingers of transcription factor IIIA.
Lee BM, Xu J, Clarkson BK, Martinez-Yamout MA, Dyson HJ, Case DA, Gottesfeld JM, Wright PE
J Mol Biol 357 275-91 (2006)
PMID: 16405997

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-527173 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription factor IIIA Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.3 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03001 (Residues: 126-212; Coverage: 24%)
Gene name: gtf3a
Sequence domains:
Structure domains: Classic Zinc Finger
55-MER Chain: B
Molecule details ›
Chain: B
Length: 55 nucleotides
Theoretical weight: 17.63 KDa
Source organism: Xenopus laevis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, molecular dynamics, generalized Born solvation model
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
10 more

12 mentions without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
11 more