› 2hko

X-ray diffraction
2.8Å resolution

Crystal structure of LSD1

Released:
DOI: 10.2210/pdb2hko/pdb
PDB entry 2hko coloured by chain, front view.
PDB entry 2hko coloured by chain, side view.
PDB entry 2hko coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human histone lysine-specific demethylase 1 (LSD1).
Chen Y, Yang Y, Wang F, Wan K, Yamane K, Zhang Y, Lei M
Proc Natl Acad Sci U S A 103 13956-61 (2006)
PMID: 16956976

Function and Biology Details

Reaction catalysed: 
(1a) a [histone H3]-N(6),N(6)-dimethyl-L-lysine(4) + acceptor + H(2)O = a [histone H3]-N(6)-methyl-L-lysine(4) + formaldehyde + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysine-specific histone demethylase 1A Chain: A
Molecule details ›
Chain: A
Length: 664 amino acids
Theoretical weight: 73.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60341 (Residues: 172-835; Coverage: 78%)
Gene names: AOF2, KDM1, KDM1A, KIAA0601, LSD1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 1 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6122
Unit cell:
a: 187.113Å b: 187.113Å c: 106.581Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

54 review citations

Regulation of histone methylation by demethylimination and demethylation.
Klose et al. (2007)
53 more

15 mentions without citation

Pharmacological targeting of the Hsp70 chaperone.
Patury et al. (2009)
14 more