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Structure of the putative mutarotase YeaD from Salmonella typhimurium: structural comparison with galactose mutarotases.

Chittori S, Simanshu DK, Savithri HS, Murthy MR
Acta Crystallogr D Biol Crystallogr 63 197-205 (2007)
Cited: 4 times
EuropePMC logo PMID: 17242513

Abstract

Salmonella typhimurium YeaD (stYeaD), annotated as a putative aldose 1-epimerase, has a very low sequence identity to other well characterized mutarotases. Sequence analysis suggested that the catalytic residues and a few of the substrate-binding residues of galactose mutarotases (GalMs) are conserved in stYeaD. Determination of the crystal structure of stYeaD in an orthorhombic form at 1.9 A resolution and in a monoclinic form at 2.5 A resolution revealed this protein to adopt the beta-sandwich fold similar to GalMs. Structural comparison of stYeaD with GalMs has permitted the identification of residues involved in catalysis and substrate binding. In spite of the similar fold and conservation of catalytic residues, minor but significant differences were observed in the substrate-binding pocket. These analyses pointed out the possible role of Arg74 and Arg99, found only in YeaD-like proteins, in ligand anchoring and suggested that the specificity of stYeaD may be distinct from those of GalMs.

Articles - 2hta mentioned but not cited (3)

  1. Structure-based function analysis of putative conserved proteins with isomerase activity from Haemophilus influenzae. Shahbaaz M, Ahmad F, Hassan MI. 3 Biotech 5 741-763 (2015)
  2. Crystallization and preliminary X-ray diffraction analysis of the putative aldose 1-epimerase YeaD from Escherichia coli. You W, Qiu X, Zhang Y, Ma J, Gao Y, Zhang X, Niu L, Teng M. Acta Crystallogr Sect F Struct Biol Cryst Commun 66 951-953 (2010)
  3. XAC4296 Is a Multifunctional and Exclusive Xanthomonadaceae Gene Containing a Fusion of Lytic Transglycosylase and Epimerase Domains. de Oliveira ACP, Ferreira RM, Ferro MIT, Ferro JA, Zamuner C, Ferreira H, Varani AM. Microorganisms 10 1008 (2022)


Articles citing this publication (1)

  1. Multiplex PCR using YeaD and 16S rRNA gene to identify major pathogens in vibriosis of Litopenaeus vannamei. Han YJ, Jo A, Kim SW, Lee HE, Kim YC, Jeong HD, Choi YH, Kim S, Cha HJ, Kim HS. Genes Genomics 41 35-42 (2019)


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