› 2hwp

X-ray diffraction
2.48Å resolution

Crystal structure of Src kinase domain in complex with covalent inhibitor PD168393

Released:
DOI: 10.2210/pdb2hwp/pdb
PDB entry 2hwp coloured by chain, front view.
PDB entry 2hwp coloured by chain, side view.
PDB entry 2hwp coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structure-guided development of affinity probes for tyrosine kinases using chemical genetics.
Blair JA, Rauh D, Kung C, Yun CH, Fan QW, Rode H, Zhang C, Eck MJ, Weiss WA, Shokat KM
Nat Chem Biol 3 229-38 (2007)
PMID: 17334377

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.74 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 251-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DJK 2 x DJK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 42.063Å b: 63.012Å c: 73.692Å
α: 100.74° β: 89.81° γ: 90.2°
R-values:
R R work R free
0.228 0.225 0.276
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Activity-based protein profiling: from enzyme chemistry to proteomic chemistry.
Cravatt et al. (2008)
20 more

1 mention without citation

'Hot' macromolecular crystals.
Koclega et al. (2009)